adds precise, computerized control functions in all three axes or in the
Z-axis only to motorized microscope stages. The principal features of
SmartFocus are shown here:
demonstration software is included in and can be accessed from the PROBE
3D demonstration software. (Note: Because stage control and image
acquisition functions require an appropriate motorized stage and frame
grabber, these functions are simulated in the demonstration software.)
continuous and programmable adjustment of Z is available in increments
locked to the working distance of the selected Objective. Selected
focus points can be directly entered into Multi-Sequence scripts.
Note access to Z-axis calibration system
stage motion can be controlled in steps or continuous motion,
under direct control or pre-programmed, with adjustable levels
of motion rate and precision. Once an image is found in the microscope,
its XY coordinates are recorded automatically for later recovery.
automatically measures full XY stage travel and calculates center
of stage. Alternative Zero coordinates can be set.
slice imaging scripting (see below) is accessed directly.
Multi-Sequence Edit dialog box provides simple scripting of a
single line of an acquisition scenario. Any number of scenario
lines can be "daisy chained" to create complex scenarios covering
multiple XY points, with multiple focused image slices at each
can be added as desired. These notes, together with calibration
and other acquisition data, are attached to the resulting image
of the microscope system using standard images with known dimensions
data is associated with the Objective used and is attached to
each subsequent image taken using that Objective.
a full demonstration of SmartFocus,